Integrated Microfluidics for Serotype Identification of Foot and Mouth Disease Virus
نویسندگان
چکیده
This work reports design and characterization results for an integrated microfluidics-based biosensor being developed for field-based Foot-and-Mouth-Disease Virus (FMDV) detection. Our laboratory is developing a field-based analysis tool for rapid FMDV serotype identification from clinical samples that range from vesicular fluid, feet or nose epithelium to whole blood. This paper outlines the specific challenges towards integration of such a device and describes coating materials and characterization of the modules with FMDV serotypes. The modules include a sample preparation module for viral RNA extraction using a disposable silica filter and on-chip RT PCR for detection with high sensitivity and specificity.
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